Taliansky, M.E. and Ryabov, E.V. and Robinson, D.J. (1998) Two distinct mechanisms of transgenic resistance mediated by groundnut rosette virus satellite RNA sequences. Molecular Plant-Microbe Interactions, 11 (5). pp. 367-374.
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Abstract
Transformation of Nicotiana benthamiana with full-length sequences of a mild variant of the groundnut rosette virus (GRV) satellite RNA (sat-RNA) yielded plants that did not produce symptoms when inoculated with GRV and a virulent sat-RNA. Two different resistance mechanisms operated in different transformed lines. In the first, plants contained high levels of transcript RNA, and replication of both sat-RNA and GRV genomic RNA was inhibited. This mechanism is analogous to the down-regulation of GRV genomic and sat-RNA replication in infections containing the mild sat-RNA, and indeed infection of sat-RNA transgenic plants with GRV was shown to lead to liberation of unit-length sat-RNA from transgene transcripts. In the second resistance mechanism, plants contained low transcript RNA levels, and replication of sat-RNA but not of GRV genomic RNA was inhibited. These plants were also resistant to infection by potato virus X derivatives containing GRV sat-RNA sequences. This mechanism is an example of homology-dependent gene silencing or cosuppression. Resistant plants were also produced by transformation with sequences representing only the 5′ terminal one-third of the mild sat-RNA; the mechanism of resistance in these plants was of the cosuppression type
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Uncontrolled Keywords: | Groundnut, rosette, disease |
| Author Affiliation: | Virology Department, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK |
| Subjects: | Plant Protection Crop Improvement > Genetics/Genomics Crop Improvement > Plant Breeding Crop Improvement > Biotechnology |
| Divisions: | Groundnut |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 19 Mar 2013 08:24 |
| Last Modified: | 01 Apr 2013 04:34 |
| Official URL: | http://dx.doi.org/10.1094/MPMI.1998.11.5.367 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9914 |
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