DNA Fingerprinting Analysis of Vegetative Compatibility Groups in Aspergillus flavus from a Peanut Field in Georgia

McAlpin, C.E. and Wicklow, D.T. and Horn, B.W. (2002) DNA Fingerprinting Analysis of Vegetative Compatibility Groups in Aspergillus flavus from a Peanut Field in Georgia. Plant Disease, 86 (3). pp. 254-258.

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Abstract

The ability of species-specific DNA probe pAF28 to correctly match 75 strains of Aspergillus flavus isolated from a peanut field in Georgia with 1 of 44 distinct vegetative compatibility groupings (VCGs) was assessed. Multiple strains belonging to the same VCG typically produced identical DNA fingerprints, with the exception of VCG 17 and VCG 24, which contained strains that showed 83 and 87% similarity, respectively. A. flavus isolates sharing more than 80% of the fragments are recognized as belonging to the same DNA fingerprint group. Each VCG represented by a single isolate produced unique DNA fingerprints. The results provide further evidence that the pAF28 probe is able to distinguish A. flavus VCGs based on DNA fingerprints and can be used to predict the approximate number of VCGs in a sample population. The DNA probe also hybridized strongly and displayed multiple and distinct bands with other species in Aspergillus section Flavi: A. bombycis, A. caelatus, A. nomius, A. pseudotamarii, and A. tamarii. Although individual strains representing Aspergillus spp. in section Flavi produced DNA fingerprints with multiple bands, the banding patterns could not be used to classify these strains according to species.

Item Type: Article
Additional Information: We thank C. Platis for assistance with the analysis of DNA fingerprints.
Uncontrolled Keywords: Aspergillus bombycis, A. caelatus, A. nomius, A. pseudotamarii, A. tamarii, cluster analysis, corn, DNA profile, restriction fragments
Author Affiliation: Mycotoxin Research Unit, National Center for Agricultural Utilization Research, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Peoria, IL 61604.
Subjects: Plant Protection
Crop Improvement > Genetics/Genomics
Divisions: Groundnut
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 15 Mar 2013 11:00
Last Modified: 15 Mar 2013 11:00
Official URL: http://dx.doi.org/10.1094/PDIS.2002.86.3.254
URI: http://eprints.icrisat.ac.in/id/eprint/9819

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