Sundaresha, S. and Manoj Kumar, A. and Rohini, S. and et al, . (2010) Enhanced protection against two major fungal pathogens of groundnut, Cercospora arachidicola and Aspergillus flavus in transgenic groundnut over-expressing a tobacco β 1–3 glucanase. European Journal of Plant Pathology, 126 (4). pp. 497-508.
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Abstract
Groundnut is an important oilseed crop of the Indian subcontinent. Yield losses due to fungal diseases are enormous in the cultivation of this crop. Over-expression of PR proteins leads to increased resistance to pathogenic fungi in several crops. The PR protein glucanase hydrolyses a major cell-wall component, glucan, of pathogenic fungi and acts as a plant defense barrier. We report in this paper, overexpression of a tobacco glucanase in transgenic groundnut and its resistance towards Cercospora arachidicola and Aspergillus flavus. PCR, Southern and Northern hybridization confirmed stable integration and expression of the glucanase gene in groundnut transgenics. When screened for resistance against Cercospora arachidicola the transgenics showed not only reduction in the number of spots but also delay in the onset of disease. Resistance was also demonstrated against one another important pathogen of groundnut, Aspergillus flavus. The transgenics not only resisted hyphal spread but also did not accumulate aflatoxin in the seeds. The results demonstrate the potential of a PR protein from a heterologous source in developing fungal disease resistant groundnut.
| Item Type: | Article |
|---|---|
| Additional Information: | We wish to acknowledge Dr. K. Veluthambi, Madurai Kamaraj University for the binary vector, pKVD4 and Dr. P. Sudhakar, Regional Agicultural Research Station, Tirupati for the Aspergillus flavus bioassay and quantification of Aflatoxin. |
| Uncontrolled Keywords: | Glucanases, Disease resistance, Groundnut, Cercospora arachidicola, Aspergillus flavus. |
| Author Affiliation: | Department of Crop physiology, University of Agricultural Sciences, G.K.V.K., Bangalore, 560 065, India |
| Subjects: | Plant Protection Crop Improvement > Genetics/Genomics Plant Physiology and Biochemistry > Plant Physiology |
| Divisions: | Groundnut |
| Depositing User: | Mr Balakrishna Garadasu |
| Date Deposited: | 15 Mar 2013 10:54 |
| Last Modified: | 15 Mar 2013 10:54 |
| Official URL: | http://dx.doi.org/10.1007/s10658-009-9556-6 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9815 |
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