The effect of glycinebetaine or ascorbic acid on grain germination and leaf structure of sorghum plants grown under salinity stress

Arafa, A.A. and Khafagy, M.A. and El-Banna, M.F. (2009) The effect of glycinebetaine or ascorbic acid on grain germination and leaf structure of sorghum plants grown under salinity stress. Australian Journal of Crop Science, 3 (5). pp. 294-304.

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Abstract

The effects of exogenously applied glycinebetaine or ascorbic acid (pre-soaking or pre-soaking plus spraying) on sorghum seedlings grown under salt stress were investigated. The seedlings were grown in hydroponic culture containing nutrient solution for 3 weeks and treated with NaCl at 1500, 3000 and 6000 ppm. Salinity increased significantly the germination percentage of sorghum grains when applied at low level but tended to decrease as the concentration of salt was raised. Ascorbic acid or glycinebetaine either alone or in combination with salinity increased germination percentage and ascorbic acid was more effective in this respect. Concerning leaf anatomy it was found that low salinity level (1500 ppm NaCl) increased the blade thickness, xylem and phloem tissues thickness and metaxylem vessel diameter as well as main vascular bundle dimensions. At the same time, moderate and high salinity levels (3000 and 6000 ppm NaCl) decreased all these parameters. The great reduction was observed under high salinity level. However, ascorbic acid or glycinebetaine had a stimulating effect in this respect and glycinebetaine proved to be more effective in this respect particularly in case of pre-soaking plus spraying method. On the other hand, salinity induced leaf cell damages in leaf sorghum plant as compared to control plants. It could be concluded that glycinebetaine or ascorbic acid applications could minimize the harmful effects of salt and pre-soaking plus spraying was found to be more effective than that of pre-soaking application.

Item Type: Article
Author Affiliation: Agriculture Botany Department, Faculty of Agriculture, Mansoura University, Mansoura, Egypt
Subjects: Soil Science and Microbiology > Soil Sciences
Divisions: Sorghum
Depositing User: David T
Date Deposited: 12 Dec 2010 17:27
Last Modified: 29 Dec 2010 19:52
Official URL: http://www.cropj.com/Khafagy2_3_5_2009_294_304.pdf
URI: http://eprints.icrisat.ac.in/id/eprint/966

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