Microbial engineering strategies to improve cell viability for biochemical production

Lo, T. and Suong, T.W. and Ling, H. and et al, . (2013) Microbial engineering strategies to improve cell viability for biochemical production. Biotechnology Advances. pp. 1-49.

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Abstract

Efficient production of biochemicals using engineered microbes as whole-cell biocatalysts requires robust cell viability. Robust viability leads to high productivity and improved bioprocesses by allowing repeated cell recycling. However, cell viability is negatively affected by a plethora of stresses, namely chemical toxicity and metabolic imbalances, primarily resulting from bio-synthesis pathways. Chemical toxicity is caused by substrates, intermediates, products, and/or by-products, and these compounds often interfere with important metabolic processes and damage cellular infrastructures such as cell membrane, leading to poor cell viability. Further, stresses on engineered cells are accentuated by metabolic imbalances, which are generated by heavy metabolic resource consumption due to enzyme overexpression, redistribution of metabolic fluxes, and impaired intracellular redox state by co-factor imbalance. To address these challenges, herein, we discuss a range of key microbial engineering strategies, substantiated by recent advances, to improve cell viability for commercially sustainable production of biochemicals from renewable resources.

Item Type:	Article
Author Affiliation:	School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore 637459, Singapore
Subjects:	Plant Protection Plant Physiology and Biochemistry > Biochemistry
Divisions:	General
Depositing User:	Mr. SanatKumar Behera
Date Deposited:	12 Feb 2013 03:45
Last Modified:	12 Feb 2013 03:45
Official URL:	http://dx.doi.org/10.1016/j.biotechadv.2013.02.001
URI:	http://eprints.icrisat.ac.in/id/eprint/9608

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