Indole-3-Acetic Acid Biosynthesis in Fusarium delphinoides Strain GPK, a Causal Agent of Wilt in Chickpea

Kulkarni, G.B. and Sanjeevkumar, S. and Kirankumar, B. and Santoshkumar, M. (2013) Indole-3-Acetic Acid Biosynthesis in Fusarium delphinoides Strain GPK, a Causal Agent of Wilt in Chickpea. Applied Biochemistry and Biotechnology . pp. 1-14.

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Abstract

Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the presence of metabolic intermediates of indole-3-pyruvic acid (IPyA), indole-3-acetamide (IAM), and tryptamine (TRA) pathways. The different IAA biosynthetic pathways were further confirmed by identifying the presence of different enzymes of these pathways. Substrate specificity study of aromatic amino acid aminotransferase revealed that the enzyme is highly specific for tryptophan (Trp) and α-ketoglutarate (α-kg) as amino group donor and acceptor, respectively. Furthermore, the concentration-dependent effect of exogenous IAA on fungal growth was established. Low concentration of exogenous IAA increases the fungal growth and at high concentration it decreases the growth of FDG.

Item Type: Article
Uncontrolled Keywords: Indole-3-acetic acid . Fusarium delphinoides . Phytopathogen . IAA biosynthetic pathway . Aminotransferase
Author Affiliation: Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India
Subjects: Plant Protection
Plant Physiology and Biochemistry > Biochemistry
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 12 Feb 2013 03:30
Last Modified: 12 Feb 2013 03:31
Official URL: http://dx.doi.org/10.1007/s12010-012-0037-6
URI: http://eprints.icrisat.ac.in/id/eprint/9590

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