Changes in protein pattern during different developmental stages of somatic embryos in chickpea

Mishra, S. and Sanyal, I. and Amla , D.V. (2012) Changes in protein pattern during different developmental stages of somatic embryos in chickpea. Biologia Plantarum, 56 (4). pp. 613-619.

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Abstract

Mature embryonic axes were used for chickpea (Cicer arietinum L.) regeneration via somatic embryogenesis. Qualitative and quantitative estimation of protein profile during somatic embryogenesis by SDS-PAGE and densitometric analysis showed differential expression of various storage proteins at different stages of somatic embryo development, which was compared with the profile of developing seeds. Total protein content in somatic embryos of chickpea increased from globular stage [2.9 μg mg-1(f.m.)] to cotyledonary stage [4.8 μg mg-1(f.m.)] and then started decreasing during onset of maturation and germination [up to 1.5 μg mg-1(f.m.)]. Differential expression of seed storage proteins, late embryogenesis abundant (LEA) proteins and proteins related with stress response were documented at different stages of somatic embryogenesis. Germinating somatic embryos showed degradation products of several seed storage proteins and the appearance of new polypeptides (76.8, 67.6, 49.9 and 34.2 kDa), which were absent during differentiation of somatic embryos. A low molecular mass (17.7 kDa) polypeptide was uniformly present during all stages of somatic embryogenesis and it may belong to a group of stress-related proteins. This study describes the expression of true seed storage proteins like legumin, vicilin, convicilin and their subunits at different stages of somatic embryogenesis, which may serve as excellent markers for embryogenic pathway of regeneration in chickpea.

Item Type: Article
Uncontrolled Keywords: Cicer arietinum, mature embryonic axes, SDS-PAGE, seed storage proteins
Author Affiliation: Plant Transgenic Laboratory, Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Lucknow-226001, India
Subjects: Plant Production
Crop Improvement
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 12 Feb 2013 03:28
Last Modified: 12 Feb 2013 03:28
Official URL: http://dx.doi.org/10.1007/s10535-012-0124-0
URI: http://eprints.icrisat.ac.in/id/eprint/9558

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