Pushpalatha, H.G. and Sudisha, J. and Geetha, N.P. and Amruthesh, K.N. and Shetty, H.S. (2011) Thiamine seed treatment enhances LOX expression, promotes growth and induces downy mildew disease resistance in pearl millet. Biologia Plantarum, 55 (3). pp. 522-527.
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Abstract
Seeds of pearl millet [Pennisetum glaucum (L). R.Br.] susceptible cv. 7042S were treated with thiamine at 5, 10, 15, 20 and 25 mM concentrations and growth promotion and downy mildew resistance were tested. Seed treatment with 20 mM thiamine resulted in 72 and 70 % disease protection under greenhouse and field conditions, respectively, and enhanced vegetative and reproductive growth parameters. Analysis of lipoxygenase (LOX) activity in inoculated pearl millet seedlings at different time intervals indicated that increased LOX activity was initiated at 3 h after inoculation (hai) and maximum activity was observed at 24 hai. Northern analysis showed that LOX mRNA transcript accumulation was higher in the resistant seedlings (cv. IP18292) than in susceptible seedlings. Thiamine seed treatment induces rapid LOX gene expression and results in significant disease protection against downy mildew disease
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | induced systemic resistance, lipoxygenase, Pennisetum glaucum, Sclerospora graminicola, transcript accumulation |
| Author Affiliation: | Department of Studies in Biotechnology, University of Mysore, Mysore-570006, Karnataka, India |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Millet |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 09 Feb 2013 14:24 |
| Last Modified: | 09 Feb 2013 14:24 |
| Official URL: | http://dx.doi.org/10.1007/s10535-011-0070-2 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9554 |
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