Nondestructive NIR Reflectance Spectroscopic Method for Rapid Fatty Acid Analysis of Peanut Seeds

Sundaram , J. and Kandala , C.V. and Butts , C.L. and Chen , C.Y. and Sobolev, V. (2011) Nondestructive NIR Reflectance Spectroscopic Method for Rapid Fatty Acid Analysis of Peanut Seeds. Peanut Science, 38 (2). pp. 85-92.

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Abstract

Near Infrared Reflectance Spectroscopy (NIRS) was used to rapidly and nondestructively analyze the fatty acid concentration present in peanut seeds samples. Absorbance spectra were collected in the wavelength range from 400 nm to 2500 nm using NIRS. The oleic, linoleic and palmitic fatty acids were converted to their corresponding methyl esters and their concentrations were measured using a gas chromatograph (GC). Partial least square (PLS) analysis was performed on a calibration set, and models were developed for prediction of fatty acid concentrations. The best model was selected based on the coefficient of determination (R2), Root Mean Square Error of Prediction, residual percent deviation (RPD) and correlation coefficient percentage between the gas chromatography measured values and the NIR predicted values. The NIR reflectance model developed yielded RPD values of three and above for prediction of the three fatty acids, indicating that this nondestructive method would be suitable for fatty acid predictions in peanut seeds.

Item Type:	Article
Uncontrolled Keywords:	Nondestructive, NIR reflectance spectroscopy, Partial Least Squares, Peanut kernels, Seeds, Fatty acids
Author Affiliation:	Agricultural Engineer, USDA, ARS, Russell Research Center, Athens, GA
Subjects:	Plant Protection Crop Improvement Plant Physiology and Biochemistry > Biochemistry
Divisions:	Groundnut
Depositing User:	Mr B Krishnamurthy
Date Deposited:	04 Feb 2013 05:09
Last Modified:	04 Feb 2013 05:09
Official URL:	http://dx.doi.org/10.3146/PS10-3.1
URI:	http://eprints.icrisat.ac.in/id/eprint/9486

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