Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTLAR1 implicated in ascochyta blight resistance in chickpea

Madrid, E. and Rajesh, P.N. and Rubio, J. and Gil, J. and Millán, T. and Chen, W. (2012) Characterization and genetic analysis of an EIN4-like sequence (CaETR-1) located in QTLAR1 implicated in ascochyta blight resistance in chickpea. Plant Cell Reports, 31 (6). pp. 1033-1042.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

Two alleles of a chickpea (Cicer arietinum L.) ethylene receptor-like sequence (CaETR-1) were sequence-characterized using synteny analysis with genome sequences of Medicago truncatula L. The full length of the sequence obtained in the accession FLIP84-92C resistant to ascochyta blight (CaETR-1a) span 4,428 bp, including the polyadenylation signal in the 3′-untranslated region (UTR), whereas it has a 730 bp deletion in the 3′-UTR region in the susceptible accession PI359075 (CaETR-1b). The deduced protein belongs to subfamily II of the ethylene receptors and contains all the domains that define EIN4 homologs in Arabidopsis. The EIN4-like sequence (CaETR-1) has been mapped using a recombinant inbred line (RIL) population derived from an intraspecific cross between ILC3279 and WR315, resistant and susceptible to blight, respectively. The locus was located in LGIVa of the genetic map, flanked by markers NCPGR91 and GAA47 (at distances of 11.3 and 17.9 cM, respectively). This is the first potentially functional sequence identified under a QTL peak for ascochyta blight resistance in chickpea (QTLAR1). This EIN4-like (CaETR-1) sequence explained up to 33.8% of the total phenotypic variation. This sequence could be directly related to blight resistance, together with other QTLs that have been found to be involved in resistance to this major chickpea disease

Item Type: Article
Uncontrolled Keywords: Chickpea, Ascochyta blight, Quantitative trait loci, Ethylene receptors
Author Affiliation: Departamento de Genética, Universidad de Córdoba, Campus Rabanales, Edif. C5, 14071 Córdoba, Spain
Subjects: Crop Improvement
Divisions: Chickpea
Depositing User: Mr B Krishnamurthy
Date Deposited: 03 Feb 2013 11:12
Last Modified: 03 Feb 2013 11:12
Official URL: http://dx.doi.org/10.1007/s00299-011-1221-9
URI: http://eprints.icrisat.ac.in/id/eprint/9405

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.