Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta

Cary, J.W. and Rajasekaran, K. and Jaynes, J.M. and Cleveland, T.E. (2000) Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta. Plant Science, 154 (2). pp. 171-181.

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Abstract

Transgenic tobacco plants producing the synthetic antimicrobial peptide D4E1, encoded by a gene under the control of an enhanced cauliflower mosaic virus 35S RNA promoter, were obtained by Agrobacterium-mediated transformation. Successful transformation was demonstrated by PCR and Southern hybridization analysis of tobacco DNAs. Expression of the synthetic D4E1 gene was shown by RT-PCR of tobacco mRNA. Crude protein extracts from leaf tissue of transformed plants significantly reduced the number of fungal colonies arising from germinating conidia of Aspergillus flavus and Verticillium dahliae by up to 75 and 99%, respectively, compared to extracts from plants transformed with pBI121. Compared to negative controls, tobacco plants expressing the D4E1 gene showed greater levels of disease resistance in planta to the fungal pathogen, Colletotrichum destructivum, which causes anthracnose.

Item Type: Article
Additional Information: The authors would like to thank Demegen, Mycogen for the D4E1 peptide and its sequence, Tony DeLucca for his advice on antifungal assays, and Maren Klich for the V. dahliae culture. We are grateful to Pamela Harris, Kurt Stromberg, and Neel Barnaby for their excellent technical assistance.
Uncontrolled Keywords: Aspergillus flavus; Colletotrichum destructivum; Disease resistance; Nicotiana tabacum; Synthetic peptide; Transgenic; Verticillium dahliae
Author Affiliation: USDA, ARS, Southern Regional Research Center, Food and Feed Safety Research Unit, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA
Subjects: Plant Production
Crop Improvement
Divisions: Other Crops
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 24 Jan 2013 10:28
Last Modified: 24 Jan 2013 10:28
Official URL: http://dx.doi.org/10.1016/S0168-9452(00)00189-8
URI: http://eprints.icrisat.ac.in/id/eprint/9383

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