Yan, Li and Liu, S and Zhao, S. and et al, . (2012) Identification of differentially expressed genes in sorghum (Sorghum bicolor) brown midrib mutants. Physiologia Plantarum, 146 (4). pp. 375-387.
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Abstract
Sorghum, a species able to produce a high yield of biomass and tolerate both drought and poor soil fertility, is considered to be a potential bioenergy crop candidate. The reduced lignin content characteristic of brown midrib (bmr) mutants improves the efficiency of bioethanol conversion from biomass. Suppression subtractive hybridization combined with cDNA microarray profiling was performed to characterize differential gene expression in a set of 13 bmr mutants, which accumulate significantly less lignin than the wild-type plant BTx623. Among the 153 differentially expressed genes identified, 43 were upregulated and 110 downregulated in the mutants. A semi-quantitative RT–PCR analysis applied to 12 of these genes largely validated the microarray analysis data. The transcript abundance of genes encoding l-phenylalanine ammonia lyase and cinnamyl alcohol dehydrogenase was less in the mutants than in the wild type, consistent with the expectation that both enzymes are associated with lignin synthesis. However, the gene responsible for the lignin synthesis enzyme cinnamic acid 4-hydroxylase was upregulated in the mutants, indicating that the production of monolignol from l-phenylalanine may involve more than one pathway. The identity of the differentially expressed genes could be useful for breeding sorghum with improved efficiency of bioethanol conversion from lignocellulosic biomass.
| Item Type: | Article |
|---|---|
| Additional Information: | This research was funded by the US Department of Agriculture, Agricultural Research Service and the National Key Technology R&D Program of China (2009BADA7B01). The authors thank Ms. Angie Phillips for her excellent technical support. The US Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability and, where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual’s income is derived from any public assistance program. (Not all prohibited bases apply to all programs). Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA’s TARGET Center at (202)720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800)795-3272 (voice) or (202)720-6382 (TDD). USDA is an equal opportunity provider and employer |
| Author Affiliation: | Shandong University, China, USDA-ARS Plant Science Research Laboratory, Stillwater, USA, Plant Stress and Germplasm Development Unit, USDA-ARS, Lubbock, TX 79415, USA |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 09 Jan 2013 09:37 |
| Last Modified: | 09 Jan 2013 09:37 |
| Official URL: | http:/dx.doi.org/10.1111/j.1399-3054.2012.01646.x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9318 |
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