Comparative study on the induction of defense related enzymes in two different cultivars of chickpea (Cicer arietinum L) genotypes by salicylic acid, spermine and Fusarium oxysporum f. sp. ciceri

Raju, S. and Jayalakshmi, S.K. and Sreeramulu, K. (2008) Comparative study on the induction of defense related enzymes in two different cultivars of chickpea (Cicer arietinum L) genotypes by salicylic acid, spermine and Fusarium oxysporum f. sp. ciceri. Australian Journal of Crop Science, 2 (3). pp. 121-140.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

Induction of some defense related enzymes and phenolics in roots and shoots of two different genotypes of chickpea cultivars which were susceptible (L550) and resistant (ICCV10) to wilt disease treated with salicylic acid, spermine (Spm), SA+Spm and Fusarium oxysporum f. sp. ciceri was investigated. Higher levels of polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), �-1, 3-glucanase (PR-2) and phenolics were observed in roots and shoots of resistant cultivar than that of susceptible cultivar on treatment with elicitors and pathogen. However, no major changes were observed in susceptible cultivar after the treatments. �-1, 3-glucanase is constitutively present and is further induced in roots and shoots of resistant cultivar by F. oxysporum f. sp. ciceri. No induction of �-1, 3- glucanase was observed in susceptible cultivar. The structural changes during disease progression were observed by histochemical staining. However, the pathogen invasion was more in susceptible cultivar compared with resistant cultivar. Further, the invasion was restricted in roots of resistant cultivar treated with SA. These results suggest that induction of defense proteins and accumulation of phenolics might have contributed to restrict the invasion of F.oxysporum f. sp. ciceri, in resistant cultivar ICCV10.

Item Type: Article
Author Affiliation: Department of Biochemistry, Gulbarga University, Gulbarga-585 106, India
Subjects: Plant Protection
Divisions: Chickpea
Depositing User: David T
Date Deposited: 08 Dec 2010 20:10
Last Modified: 29 Dec 2010 19:57
Official URL: http://www.cropj.com/Microsoft%20Word%20-%20Main%2...
URI: http://eprints.icrisat.ac.in/id/eprint/931

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.