Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. ciceri.

Jayalakshmi, S.K. and Raju, S. and Rani, S. Usha and Benagi, V.I. and Sreeramulu, K. (2009) Trichoderma harzianum L1 as a potential source for lytic enzymes and elicitor of defense responses in chickpea (Cicer arietinum L.) against wilt disease caused by Fusarium oxysporum f. sp. ciceri. Australian Journal of Crop Science, 3 (1). pp. 44-52.

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Abstract

The effect of some natural lignocellulosic substances on the production of �-glucanase, chitinase, protease and xylanase from Trichoderma harzianum L1 has been studied under solid state fermentation conditions. Maximum activities of all these enzymes were observed in the fermentation medium containing the mixture of 1% rice bran, neem cake and 0.1% crab shell powder. The induction of plant defense response was investigated by inoculating the roots of chickpea cv JG62 with the biocontrol agent, T. harzianum L1. A root extract of chickpea inoculated with T. harzianum L1 showed increased activities of phenylalanine ammonia lyase and polyphenol oxidase, as well as induction of new trypsin and chymotrypsin inhibitors. The Fusarium oxysporum protease-2 was inhibited completely by root extract of chickpea inoculated with T. harzianum L1 and showed maximum resistance to rotting of roots caused by wilt disease

Item Type: Article
Author Affiliation: Department of Biochemistry, Gulbarga University, Gulbarga-585 106, India
Subjects: Crop Improvement > Plant Breeding
Divisions: Chickpea
Depositing User: David T
Date Deposited: 08 Dec 2010 20:12
Last Modified: 29 Dec 2010 19:57
Official URL: http://www.cropj.com/Sreer_January2009_44_52.pdf
URI: http://eprints.icrisat.ac.in/id/eprint/930

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