Iron transport and storage within the seed coat and embryo of developing seeds of pea (Pisum sativum

Marentesi, E. and Grusak, M.A. (1998) Iron transport and storage within the seed coat and embryo of developing seeds of pea (Pisum sativum. Seed Science Research, 8 (3). pp. 367-375.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

To understand the cellular processes related to iron transport and sequestration within the developing pea seed (Pisum sativum), total iron and ferritin iron were analysed in seed coat and embryo tissues of the iron-hyperaccumulating pea mutant, Sparkle [dgl, dgl], and its wild-type parent, cv. Sparkle. For plants grown hydroponically with 2 um Fe, embryo Fe concentrations averaged 65 pg g-1 dry weight in mature wild-type seeds and 163 pg g--1 dry weight in mature dgl seeds; iron concentrations were also higher in dgl seed coats. Extracted and electrophoretically separated seed proteins were probed with a polyclonal antibody raised against pea seed ferritin. In both genotypes, ferritin was detected in the embryo, but not in the seed coat. Ferritin iron accounted for 92% of the total iron in mature wild-type embryos, but only 42% of the total iron in mature dgl embryos. Radiotracer studies using "Fe were used to characterize the movement of iron within the seed coat.

Item Type: Article
Additional Information: This research was funded in part with federal funds from the US Department of Agriculture, Agricultural Research Service under Co-operative Agreement number 58-6250-1-003 and from the US Department of Agriculture, Co-operative State Research Service under Agreement number 94-37100-0823 to M.A.G.
Uncontrolled Keywords: iron, ferritin, seed development, seed coat, embryo, phloem unloading, Pisum sativum L.
Author Affiliation: Department of Biological Sciences, Biological Science Centre, University of Alberta, Edmonton, Alberta, T6G 2E9, Canada
Subjects: Crop Improvement > Seed Technology
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 04 Jan 2013 06:11
Last Modified: 04 Jan 2013 06:11
Official URL: http://dx.doi.org/10.1017/S0960258500004293
URI: http://eprints.icrisat.ac.in/id/eprint/9295

Actions (login required)

View Item View Item
EPrints Logo
eprints is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.