Highly variable ‘Arhar’ simple sequence repeat markers for molecular diversity and phylogenetic studies in pigeonpea [Cajanus cajan (L.) Millisp.]

Dutta, S. and Mahato, A.K. and Sharma, P. and Singh, N.K and et al, . (2012) Highly variable ‘Arhar’ simple sequence repeat markers for molecular diversity and phylogenetic studies in pigeonpea [Cajanus cajan (L.) Millisp.]. Plant Breeding. pp. 1-6.

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Abstract

Pigeonpea is an important tropical grain legume widely cultivated in South and South-East Asia for versatile end usage as food, feed, fodder and fuel. Recent publication of draft genome sequence of pigeonpea (‘Arhar’) has allowed mining of large numbers of genomic simple sequence repeat (SSR) markers most of which are either not validated or show very little polymorphism in simple agarose gel–based assays. Here, we describe a special category of 370 validated highly variable ‘Arhar’ genomic SSR (HASSR) markers, which provide much higher level of polymorphism than a random set of SSR markers. These markers were validated for consistent amplification in a set of eight pigeonpea varieties and 152 of these (41%) showed allelic polymorphism on agarose gel electrophoresis. Twenty-four highest polymorphic HASSR markers were used on a larger set of forty pigeonpea varieties and eight wild Cajanus species for the analysis of genetic diversity and phylogenetic relationship. Genomic HASSR markers described here are highly suitable for genetic diversity and phylogenic studies in pigeonpea.

Item Type: Article
Additional Information: We are grateful to Indian Council of Agricultural Research, New Delhi for financial support of Network Project on Transgenics in Crops (NPTC) project. SD is grateful to Council of Scientific and Industrial Research, Government of India for financial support (Grant no. 09/083/ (0342)/2011/EMR-I).
Uncontrolled Keywords: pigeonpea, SSR marker, polymorphism, diversity
Author Affiliation: National Research Centre on Plant Biotechnology, New Delhi, India
Subjects: Crop Improvement > Plant Breeding
Divisions: Pigeonpea
Depositing User: Mr Siva Shankar
Date Deposited: 28 Dec 2012 08:15
Last Modified: 28 Dec 2012 08:15
Official URL: http://dx.doi.org/10.1111/pbr.12014
URI: http://eprints.icrisat.ac.in/id/eprint/9243

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