SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing

Lindner, H. and Raissig, M.T. and Sailer, C. and et al, . (2012) SNP-Ratio Mapping (SRM): Identifying Lethal Alleles and Mutations in Complex Genetic Backgrounds by Next-Generation Sequencing. Genetics, 191 (4). pp. 1381-1386.

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Abstract

We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.

Item Type: Article
Author Affiliation: Institute of Plant Biology and Zürich-Basel Plant Science Center, University of Zürich, CH-8008 Zürich, Switzerland
Subjects: Crop Improvement > Genetics/Genomics
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 28 Dec 2012 07:31
Last Modified: 28 Dec 2012 07:31
Official URL: http://dx.doi.org/10.1534/genetics.112.141341
URI: http://eprints.icrisat.ac.in/id/eprint/9241

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