Young, N.D.
(1994)
Constructing a plant genetic linkage map with DNA markers.
In:
DNA-based markers in plants.
Advances in Cellular and Molecular Biology of Plants, 1
.
Kluwer Academic, Dordrecht, pp. 39-57.
ISBN 0-7923-2714-4
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Abstract
Scientists are constructing genetic linkage maps composed of DNA markers for a wide range of plant species (O’Brien, 1993). Several types of DNA markers have been widely
used (Fig. 1): restriction fragment length polymorphisms (RFLPs) (Botstein et al. 1980), random amplified polymorphic DNAs (RAPDs) (Williams et al. 1990), simple sequence repeats (SSRs or microsatellites) (Litt and Luty 1989) and amplified fragment length polymorphisms (AFLPs) (Vos et al. 1995). In the future, maps built from single nucleotide polymorphisms (SNPs) in combination with DNA chip technology are likely (Wang et al. 1998). All types of DNA markers detect sequence polymorphisms and monitor the segregation of a DNA sequence among progeny of a genetic cross in order
to construct a linkage map. While the theory of linkage mapping is the same for DNA markers as in classical genetic mapping, special considerations must be kept in mind. This is primarily a result of the fact that potentially unlimited numbers of DNA markers can be analyzed in a single mapping population. Backcross and F2 populations are suitable for DNA-based mapping, but recombinant inbred (Burr and Burr, 1991) and doubled haploid lines (Huen et al. 1991) provide permanent mapping resources. These types of populations are also better suited for analysis of quantitative traits.
| Item Type: |
Book Section
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| Additional Information: |
This paper is published as a contribution of the series of the Minnesota Agricultural Experiment Station on research conducted under Project 015, supported by G.A.R. funds. |
| Uncontrolled Keywords: |
Plant genetic linkage map, DNA markers |
| Author Affiliation: |
Department of Plant Pathology, University of Minnesota, USA. |
| Subjects: |
Crop Improvement |
| Divisions: |
General |
| Depositing User: |
Mr Arbind Seth
|
| Date Deposited: |
01 Jan 2013 11:21 |
| Last Modified: |
01 Jan 2013 11:21 |
| Official URL: |
http://d.yimg.com/kq/groups/18463231/399330215/nam... |
| URI: |
http://eprints.icrisat.ac.in/id/eprint/9150 |
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