Gomez, S.M. and Denwar, N.N. and Ramasubramanian, T. and et al, . (2008) Identification of peanut hybrids using microsatellite markers and horizontal polyacrylamide gel electrophoresis. Peanut Science , 35 (2). pp. 123-129.
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Abstract
In peanut hybridization, distinguishing inadvertent selfs from the true hybrids may be difficult. In this study, to differentiate between selfs and hybrids, DNA was extracted from leaf tissue of F1 or F2 plants, and SSR markers were amplified and bands separated by a novel submarine horizontal polyacrylamide gel electrophoresis (H-PAGE). By comparing the resulting banding patterns to those of the parents, 70% of the putative hybrids were shown to be true hybrids on the basis of possessing a marker allele from the male parent. The H-PAGE gels gave better band separation and differentiation of selfed progenies than agarose gels, and were compatible with the common horizontal agarose gel units. This method provides a quick assay to distinguish hybrids from inadvertent selfs, and should result in greater efficiency and more effective use of resources in peanut breeding programs.
| Item Type: | Article |
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| Additional Information: | The authors would like to express thanks to Jamie Ayers and Yolanda Lo´pez for assistance with greenhouse work. Funding for these studies was provided by the National Peanut Board, the Southwest Consortium for Plant Genetics and Water Resources (#2004-34186-14533), and the Peanut Collaborative Research Support Project. |
| Uncontrolled Keywords: | Hybridization, Heterozygote identification, Pollination, SSR markers. |
| Author Affiliation: | Department of Plant and Soil Science, Texas Tech University, USA. |
| Subjects: | Crop Improvement Crop Improvement > Plant Breeding |
| Divisions: | Groundnut |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 01 Jan 2013 10:54 |
| Last Modified: | 01 Jan 2013 10:54 |
| Official URL: | http://naldc.nal.usda.gov/download/35140/PDF |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9146 |
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