Hu , D. and Li , M. and Luo , H. and et al, . (2012) Molecular cloning and functional characterization of MdSOS2 reveals its involvement in salt tolerance in apple callus and Arabidopsis. Plant Cell Reports , 31 (4). pp. 713-722.
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Abstract
Plants respond to various environmental stresses by activating "stress genes". CIPKs (CBL-interacting protein kinases) family genes play an important role in the process of stress response. In this study, a CIPK gene MdSOS2 was isolated from apple (Malus × Domestica). Sequence alignment and phylogenetic analysis showed that it is highly similar with Arabidopsis AtSOS2 and contained the conserved domains and motifs. Expression analysis demonstrated that MdSOS2 expressed in all tested organs at different levels, and positively in response to salt stress. Furthermore, the ectopic expression of MdSOS2 complemented the function of Arabidopsis sos2 mutant, and conferred enhanced salt tolerance to the transgenic Arabidopsis. Yeast two-hybrid assay indicated that the N-terminal of MdSOS2 protein physically interacted with MdSOS3 and AtSOS3, respectively, suggesting that SOS pathway operates in apple tree. Finally, MdSOS2 overexpression enhanced, while its suppression reduced the tolerance to salt in transgenic apple calluses, indicating that MdSOS2 acts as a positive regulator in response to salt stress in apple.
| Item Type: | Article |
|---|---|
| Additional Information: | We thank Dr. Jian-Kang Zhu for providing Arabidopsis sos2 mutant. This work was supported by the National High Technology Research and Development Program of China, 948 Project from Ministry of Agriculture (2011-G21), and ‘‘Taishan Scholar’’ project from Shandong Province of China. |
| Uncontrolled Keywords: | Apple, CIPK family, MdSOS2, MdSOS2-MdSOS3, Protein kinase complex, SOS pathway, Salt tolerance |
| Author Affiliation: | National Key Laboratory of Crop Science, National Research Center for Apple Engineering and Technology, College of Horticulture Science and Engineering, Shandong Agricultural University, China. |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 12 Dec 2012 12:09 |
| Last Modified: | 12 Dec 2012 12:09 |
| Official URL: | http://dx.doi.org/10.1007/s00299-011-1189-5 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9065 |
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