Prabu , G. and Prasad, D.T. (2012) Functional characterization of sugarcane MYB transcription factor gene promoter (PScMYBAS1) in response to abiotic stresses and hormones. Plant Cell Reports, 31 (4). pp. 661-669.
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Abstract
The sugarcane (Saccharum officinarum) stress-related MYB transcription factor gene, ScMYBAS1, demonstrated induced response to water deficit and salt stress in our previous study. To elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (PScMYBAS1, 1,033 bp) flanking the 5′ ScMYBAS1 coding region from the sugarcane genome. A series of PScMYBAS1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -843, -1,033) was fused to the uidA reporter gene (GUS) and each deletion construct was analyzed by Agrobacterium-mediated transient transformation in tobacco leaves subjected to dehydration, salinity, cold, wounding, gibberellic acid (GA), salicylic acid (SA), and methyl jasmonic acid (MeJA). Deletion analysis of the promoter, PScMYBAS1, suggested that the 303-bp promoter region was required for basal expression. Promoter fragments, 777 bp or longer showed ~twofold to ~fourfold increased induction of GUS in response to abiotic stress (dehydration, salt, cold, wounding) and hormone (SA, MeJA) treatments. These findings further our understanding of the regulation of ScMYBAS1 expression and provide a new stress-inducible promoter system in transgenic plants.
| Item Type: | Article |
|---|---|
| Additional Information: | The authors are thankful to Vasantdada Sugar Institute (VSI), India, for funding the work, Dr. S. Anandhan, DARL, Defense Research and Developmental Organization, Haldwani, India, for his valuable suggestions during the research work and Viswanathan Satheesh, Indian Agricultural Research Institute, India, for editing the manuscript. |
| Uncontrolled Keywords: | Agroinfiltration, Dehydration, Hormone, Promoter, ScMYBAS1, Sugarcane |
| Author Affiliation: | Molecular Biology and Genetic Engineering Division, Vasantdada Sugar Institute, India. |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 12 Dec 2012 12:08 |
| Last Modified: | 12 Dec 2012 12:08 |
| Official URL: | http://dx.doi.org/10.1007/s00299-011-1183-y |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9064 |
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