Gupta, N.C. and Jain, P.K. and Bhat, S.R. and Srinivasan, R. (2012) Upstream sequence of fatty acyl-CoA reductase (FAR6) of Arabidopsis thaliana drives wound-inducible and stem-specific expression. Plant Cell Reports, 31 (5). pp. 839-850.
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Abstract
An Arabidopsis mutant line T90, exhibiting a stem-specific and wound-responsive GUS expression was identified from a population of Arabidopsis thaliana tagged with a promoterless β-glucuronidase (GUS) in the T-DNA. Sequence flanking the insertion from the right border was amplified by TAIL PCR and cloned. The insertion was located in the third chromosome, 57 bp upstream of the ATG start codon in 5′ untranslated region (UTR) of the fatty acyl-CoA reductase 6 (FAR6) gene. RT-PCR analysis of the FAR6 gene revealed that the gene is expressed predominantly in stem tissue. Semi-quantitative RT-PCR showed that the expression is also induced by wounding in the epidermal layer of mature stem internodes. The transcription initiation site (TSS) was identified by 5′ RACE PCR. Different 5′ deletion fragments of the promoter sequences were developed and linked to the GUS reporter gene as transcriptional fusions and the expression patterns of GUS were histochemically analyzed in transgenic Arabidopsis plants. Sequences from -510 bp upstream to the transcriptional start site were sufficient to exhibit wound-inducible GUS expression in the stems. The addition of further upstream sequences (-510 to -958, -1,400 or -1,456) enhanced and extended the wound-inducible GUS expression throughout the mature stem.
| Item Type: | Article |
|---|---|
| Additional Information: | We acknowledge Dr. K.V. Prabhu and the staff of National Phytotron Facility, IARI, New Delhi-110012, India, for their help in growing Arabidopsis in the growth chambers and greenhouses. We also acknowledge Dr. G.J. Randhawa for providing facility for fluorometric analysis. Financial support from Department of Biotechnology, Ministry of Science and Technology, India, and Council of Scientific and Industrial Research, India, is gratefully acknowledged. |
| Uncontrolled Keywords: | Arabidopsis, Wound inducible, Stem-specific, Promoter, Fatty acyl-CoA reductase |
| Author Affiliation: | National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, India. |
| Subjects: | Plant Physiology and Biochemistry > Plant Physiology |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 12 Dec 2012 12:01 |
| Last Modified: | 12 Dec 2012 12:01 |
| Official URL: | http://dx.doi.org/10.1007/s00299-011-1205-9 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9061 |
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