Zhao , W. and Wang , S. and Li , X. and et al, . (2012) Molecular cloning and characterization of the light-regulation and circadian-rhythm of the VDE gene promoter from Zingiber officinale. Plant Cell Reports, 31 (8). pp. 1381-1392.
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Abstract
Ginger (Zingiber officinale Rosc.) is prone to photoinhibition under intense sunlight. Excessive light can be dissipated by the xanthophyll cycle, where violaxanthin de-epoxidase (VDE) plays a critical role in protecting the photosynthesis apparatus from the damage of excessive light. We isolated ~2.0 kb of ginger VDE (GVDE) gene promoter, which contained the circadian box, I-box, G-box and GT-1 motif. Histochemical staining of Arabidopsis indicated the GVDE promoter was active in almost all organs, especially green tissues. β-glucuronidase (GUS) activity driven by GVDE promoter was repressed rather than activated by high light. GUS activity was altered by hormones, growth regulators and abiotic stresses, which increased with 2,4-dichlorophenoxyacetic acid and decreased with abscisic acid, salicylic acid, zeatin, salt (sodium chloride) and polyethylene glycol. Interestingly, GUS activities with gibberellin or indole-3-acetic acid increased in the short-term (24 h) and decreased in the long-term (48 and 72 h). Analysis of 5′ flank deletion found two crucial functional regions residing in -679 to -833 and -63 to -210. Northern blotting analysis found transcription to be regulated by the endogenous circadian clock. Finally, we found a region necessary for regulating the circadian rhythm and another for the basic promoter activity.
| Item Type: | Article |
|---|---|
| Additional Information: | We give special thanks to Prof. Jun-ping Gao(China Agricultural University) for providing the vector PBI 121. We are grateful to Zheng Tong (China Agricultural University) for aiding us with experimental protocols. Funding was provided by the Natural Science Foundation of China (30771465). |
| Uncontrolled Keywords: | Ginger, VDE promoter, Arabidopsis, Abiotic stress, Circadian rhythms, Light regulation |
| Author Affiliation: | College of Agronomy and Biotechnology, China Agricultural University, China. |
| Subjects: | Crop Improvement Crop Improvement > Genetics/Genomics |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 12 Dec 2012 09:48 |
| Last Modified: | 12 Dec 2012 09:48 |
| Official URL: | http://dx.doi.org/10.1007/s00299-012-1254-8 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9045 |
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