Kato, T. and Ohta, H. and Tanaka, K. and Shibata, D. (1992) Appearance of new lipoxygenases in soybean cotyledons after germination and evidence for expression of a major new lipoxygenase gene. Plant Physiology, 98 (1). pp. 324-330.
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Abstract
The appearance and subsequent disappearance of lipoxygenase activity at pH 6.8 was observed in germinated cotyledons of variant cv. Kanto 101 that lacks the two lipoxygenase isoenzymes, L-2 and L-3, present in dry seeds of normal cv. Enrei. Three new lipoxygenases, designated lipoxygenase L-4, L-5 and L-6, were purified using anionic or cationic ion exchange chromatography. The major lipoxygenase in 5-day-old cotyledons of the variant soyabean was lipoxygenase L-4. Lipoxygenases L-5 and L-6 preferentially produced 13(S)-hydroperoxy-9(Z),11(E)-octadecadienoic acid (13S-HPOD) as a reaction product of linoleic acid, whereas lipoxygenase L-4 produced both 13S-HPOD and 9(S)-hydroperoxy-10(E),12(Z)-octadecadienoic acid. All three isoenzymes have pH optima of 6.5, no activity at pH 9.0, and preferred linolenic acid to linoleic acid as a substrate. Partial amino acid sequencing of lipoxygenase L-4 showed that this isoenzyme shares amino acid sequence homology with lipoxygenases L-1, L-2, and L-3 but is not identical to any of them. This indicated that a new lipoxygenase, L-4, is expressed in cotyledons.
| Item Type: | Article |
|---|---|
| Additional Information: | We thank Dr. Keisuke Kitamura for the gift of seeds of the lipoxygenase variant cultivar (Kanto 101). We wish to express our thanks to Drs. Tae K. Park and Joseph C. Polacco for the opportunity to see the nucleotide sequence of pTK18 in advance of publication.We also thank Dr. Bernard Axelrod for the gift of antilipoxygenase L-I antibody. We thank Yumiko Shirano for drawing figures. |
| Uncontrolled Keywords: | Lipoxygenases in Soybean,Cotyledons after Germination |
| Author Affiliation: | Mitsui Plant Biotechnology Research Institute, Japan. |
| Subjects: | Crop Improvement |
| Divisions: | Soyabean |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 12 Dec 2012 09:55 |
| Last Modified: | 12 Dec 2012 09:55 |
| Official URL: | http://dx.doi.org/10.1104/pp.98.1.324 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/9044 |
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