Shridhar, J. and Bhat, R.S. and Bhat, S. and Kuruvinashetti, M.S. (2010) Agrobacterium-mediated transformation studies in sorghum using an improved gfp reporter gene. Journal of SAT Agricultural Research, 8. 5pp..
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Abstract
Agrobacterium-mediated transformation of M 35-1, a rabi (postrainy season) sorghum cultivar, was envisaged with pKU352NA vector containing SgfpS65T, an improved gfp reporter gene and iAc encoding transposase. Among various explants tried, immature inflorescence gave the highest callus induction (81.9%). pKU352NAcarrying Agrobacterium was induced by acetosyringone (200 µM) and used for Agroinfection followed by threeday co-cultivation of 15-day-old calli. Co-cultivated calli were washed with cefotaxime (200 mg L-1) for 8 min and transferred to the regeneration medium with hygromycin (20 mg L-1). In total, 25 putative transgenic plants were obtained, of which nine survived later. When eight plants were tested with iAc-specific PCR, six yielded an expected 541bp amplicon indicating a transformation efficiency of 4.28%. SgfpS65T expression was evident in the leaves of all six iAc-specific PCR positive plants.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biotechnology, University of Agricultural Sciences (UAS), Dharwad 580 005, Karnataka, India |
| Subjects: | Plant Production |
| Divisions: | Sorghum |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 07 Dec 2010 17:52 |
| Last Modified: | 29 Dec 2010 20:01 |
| Official URL: | http://ejournal.icrisat.org/Volume8/Sorghum_Millet... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/899 |
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