Dussert, Y. and Remigereau, M.-s. and Fontaine, M.C. and et al, . (2012) Polymorphism pattern at a miniature inverted-repeat transposable element locus downstream of the domestication gene Teosinte-branched1 in wild and domesticated pearl millet. Molecular Ecology. pp. 1-14.
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Abstract
Unravelling the mechanisms involved in adaptation to understand plant morphological evolution is a challenging goal. For crop species, identification of molecular causal polymorphisms involved in domestication traits is central to this issue. Pearl millet, a domesticated grass mostly found in semi-arid areas of Africa and India, is an interesting model to address this topic: the domesticated form shares common derived phenotypes with some other cereals such as a decreased ability to develop basal and axillary branches in comparison with the wild phenotype. Two recent studies have shown that the orthologue of the maize gene Teosinte-Branched1 in pearl millet (PgTb1) was probably involved in branching evolution during domestication and that a miniature inverted-repeat transposable element (MITE) of the Tuareg family was inserted in the 3′ untranslated region of PgTb1. For a set of 35 wild and domesticated populations, we compared the polymorphism patterns at this MITE and at microsatellite loci. The Tuareg insertion was nearly absent in the wild populations, whereas a strong longitudinal frequency cline was observed in the domesticated populations. The geographical pattern revealed by neutral microsatellite loci clearly demonstrated that isolation by distance does not account for the existence of this cline. However, comparison of population differentiation at the microsatellite and the MITE loci and analyses of the nucleotide polymorphism pattern in the downstream region of PgTb1 did not show evidence that the cline at the MITE locus has been shaped by selection, suggesting the implication of a neutral process. Alternative hypotheses are discussed.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was funded by the Agence Nationale pour la Recherche Scientifique projects PLANTADIV (ANR-07Biodiv-005-04) and TRANSBIODIV (ANR-06Bdiv003-05). YD is the recipient of a PhD grant from Universite Pierre et Marie Curie Paris VI. |
| Uncontrolled Keywords: | cline;domestication;microsatellite;MITE;pearl millet; Teosinte-Branched1 |
| Author Affiliation: | Laboratoire Ecologie, Systématique et Evolution UMR 8079 CNRS, Université Paris-Sud, Orsay, France |
| Subjects: | Crop Improvement |
| Divisions: | Millet |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 06 Dec 2012 09:49 |
| Last Modified: | 06 Dec 2012 09:52 |
| Official URL: | http://dx.doi.org/10.1111/mec.12139 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8987 |
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