Varalaxmi, Y. and Vijayalakshmi, A. and Ravi Kumar, K. and Vijayalakshmi, T. and Maheswari, M. (2010) Efficient plant regeneration from shoot apices of pearl millet (Pennisetum americanum (L.) Leeke). Plant Tissue Culture and Biotechnology , 20 (1). pp. 47-53.
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Abstract
An efficient and rapid regeneration protocol was developed from shoot tip explants of Pennisetum americanum (L.) Leeke variety WC-75. MS supplemented with low concentration of 2,4-D and Kn was most effective in producing embryogenic calli. Maximum regeneration potential of 40 shoots per calli were obtained when transferred to regeneration medium containing 1.0 mg/l Kn. Shoots developed were efficiently rooted within 15 days on the medium containing NAA. Over 90% of rooted plants were fertile after transfer to a net house.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Division of Crop Sciences, Central Research Institute for Dryland Agriculture, Santhoshnagar, Hyderabad, India |
| Subjects: | Plant Protection Plant Protection > Pests |
| Divisions: | UNSPECIFIED |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 07 Dec 2010 17:30 |
| Last Modified: | 29 Dec 2010 20:01 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/895 |
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