Determination of oligosaccharide contents in 19 cultivars of chickpea (Cicer arietinum L) seeds by high performance liquid chromatography

Xiaoli, X. and Liyi, Y. and Shuang, H. and et al, . (2008) Determination of oligosaccharide contents in 19 cultivars of chickpea (Cicer arietinum L) seeds by high performance liquid chromatography. Food Chemistry, 111 (1). pp. 215-219.

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Abstract

The extraction procedure of oligosaccharides from chickpea seeds was optimized and sufficient extraction was achieved with 50% of ethanol-water in a ratio of 10:1 for solvent to defatted chickpea meal at 50 �C for 30 min. Under the optimal extraction conditions, the contents of oligosaccharides in 19 cultivars of chickpea seeds were determined by HPLC. The results showed that the main a-galactooligosaccharides (a-GOS) in chickpea seeds were raffinose, stachyose, verbascose and an unknown glycoside, which was isolated, purified, and identified as ciceritol. Ciceritol, the main sugar in all the chickpea samples, accounted for about 50% of the total a-GOS. There were considerable variations in the levels of a-GOS and sucrose between species, especially verbascose which could only be detected in 7 samples. With the highest amount of a-GOS and a low amount of sucrose, the 171 was the best choice for obtaining a-GOS for use as a prebiotic in functional foods.

Item Type: Article
Additional Information: This work was supported by a grant-in-aid from Nanjing Agricultural University for the Introduction of Outstanding Scholars (804066).
Uncontrolled Keywords: Chickpea (Cicer arietinum L) seed, a-Galactooligosaccharide, Sucrose, Optimization, Extraction method, HPLC
Author Affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China
Subjects: Postharvest Management > Food Technology
Divisions: Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 29 Nov 2012 10:04
Last Modified: 29 Nov 2012 10:04
Official URL: http://dx.doi.org/10.1016/j.foodchem.2008.03.039
URI: http://eprints.icrisat.ac.in/id/eprint/8934

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