Determination of Mono-, Di-, and Oligosaccharides in Legumes by High-Performance Liquid Chromatography Using an Amino-Bonded Silica Column

Sánchez-Mata, M.C. and Peñuela-Teruel, M.J. and Cámara-Hurtado, M. and et al, . (1998) Determination of Mono-, Di-, and Oligosaccharides in Legumes by High-Performance Liquid Chromatography Using an Amino-Bonded Silica Column. Journal of Agricultural Food Chemistry, 46 (9). pp. 3648-3652.

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Abstract

A high-performance liquid chromatography (HPLC) method for soluble sugars analysis, using an [[(aminopropyl)methyl]silyl]-bonded amorphous silica column, was applied to several dry legumes:  chickpeas, lentils, white beans, pinto beans, and peas. Monosaccharide (ribose, fructose, glucose, and galactose), disaccharide (sucrose, maltose, and melibiose), and oligosaccharide (raffinose, ciceritol, and stachyose) composition of these samples was analyzed, with special interest on α-galactosides, because of their physiological role in inducing flatulent phenomena in humans after ingestion of legumes. Ciceritol (an inositol digalactoside) was the main sugar in chickpea samples and it was present also in lentils. In all the samples stachyose was found at higher levels than raffinose, and the content of these two flatulence-inducing sugars is higher in beans and lentils and lower in peas and chickpeas. However, great variability has been found in the sugar content of the different samples analyzed, probably due to genetic and environmental factors. The proposed method showed good results for the analysis of 10 simple and complex sugars in legumes, in a final time of about 40 min and in the same chromatographic run.

Item Type: Article
Uncontrolled Keywords: Soluble sugars; oligosaccharides; R-galactosides; legumes; HPLC
Author Affiliation: Departmento de Nutrición y Bromatología II, Bromatología, Facultad de Farmacia, Universidad Complutense de Madrid, Ciudad Universitaria, E-28040 Madrid, Spain
Subjects: Postharvest Management > Food Technology
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 29 Nov 2012 04:53
Last Modified: 29 Nov 2012 04:53
Official URL: http://dx.doi.org/10.1021/jf980127w
URI: http://eprints.icrisat.ac.in/id/eprint/8931

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