McClung, C.R. and Berkum, P. Van and Davis, R.E. and Sloger, C. (1983) Enumeration and localization of N2-fixing bacteria associated with roots of Spartina alterniflora Loisel. Applied and Environmental Microbiology, 45 (6). pp. 1914-1920.
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Abstract
N2-fixing bacteria were recovered from Spartina alterniflora roots and from the surrounding salt-marsh sediment; they formed a greater proportion of the bacteria recovered from root homogenates than from salt-marsh sediment. N2-fixing bacteria were recovered in large numbers from the rhizoplane of S. alterniflora after roots were treated with 1 or 5% chloramine-T for 1 h or with 1% NaOCl for 1 or 2 h. Immersing S. alterniflora roots in 5% NaOCl for 1 h was more effective in distinguishing bacteria inside the roots, since this treatment nearly eliminated N2-fixing bacteria recoverable from the rhizoplane while large numbers of N2-fixing bacteria were recovered from root homogenates. However, this treatment was less effective with roots of Zea mays and Sorghum bicolor. Bacteria were observed by light and electron microscopy inter- and intracellularly in the cortex and in the aerenchyma of S. alterniflora roots.ADDITIONAL ABSTRACT:Estimates of both the rhizoplane and total bacterial populations associated with roots of S. alterniflora were significantly reduced by treating roots with chloramine-T or 1% NaOCl. Treatment with 5% NaOCl for 1 h nearly eliminated the recovery of N-fixing bacteria from the rhizoplane of S. alterniflora roots but was less effective for maize and sorghum roots. It is concluded that root surface sterilization techniques must be evaluated before using with different plants
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Plant Pathology, Cook College, Rutgers-The State University of New Jersey, New Brunswick, New Jersey 08903, USA |
| Subjects: | Plant Production Soil Science and Microbiology > Soil Sciences Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 21 Nov 2012 10:59 |
| Last Modified: | 21 Nov 2012 10:59 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8853 |
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