Firoozabady, E. and Moy, Y. and Courtney-Gutterson, N. and et al, . (1994) Regeneration of Transgenic Rose (Rosa hybrida) Plants from Embryogenic Tissue. Nature Biotechnology, 12. pp. 609-613.
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Abstract
Friable embryogenic tissues (FET) were recovered from filament cultures of rose, Rosa hybrida cv. Royalty, and cocultured with Agrobacterium tumefaciens or A. rhizogenes harboring a vector containing neomycin phosphotransferase II (NPTII) and -glucuronidase (GUS) or firefly luciferase (LUC) genes. Putative transformed colonies were selected on kanamycin. Fifty–60 transformed embryogenic callus lines were reproducibly obtained from each gram of FET inoculated with Agrobacterium. Transformed embryogenic calli were transferred to maturation media to form somatic embryos, which subsequently produced flowering plants. The transgenic nature of the plants was confirmed by enzyme assays, polymerase chain reaction, and Southern hybridization. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. This procedure facilitates introduction of desirable genes, especially those controlling flower color, into commercial cultivars of rose.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was primarily funded by Florigene BV, The Netherlands. |
| Uncontrolled Keywords: | Transgenic Rose |
| Author Affiliation: | DNA Plant Technology Corporation, 6701 San Pablo Ave. Oakland, California 94608 |
| Subjects: | Crop Improvement > Biotechnology |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 21 Nov 2012 03:19 |
| Last Modified: | 21 Nov 2012 03:19 |
| Official URL: | http://dx.doi.org/10.1038/nbt0694-609 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8832 |
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