Takashima, M. and Nara, K. and Niki, E. (2012) Evaluation of biological activities of a groundnut (Apios americana Medik) extract containing a novel isoflavone. Food Chemistry. pp. 1-43.
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Abstract
Groundnut (Apios americana Medik) contains a novel isoflavone, genistein-7-O-gentiobioside. In the present study, we examined the biological activities of an alcohol extract of groundnut containing genistein-7-O-gentiobioside as the main component. Although the groundnut extract by itself did not show antioxidative activity, it drove the antioxidative system in cells. Pretreatment of human breast carcinoma MCF-7 cells for 24 h with the groundnut extract and soybean isoflavone increased gene expression of heme oxygenase-1 (HO-1), a major antioxidative stress enzyme. These groundnut extract-treated cells showed antioxidative activity against free radicals derived from a radical initiator. Pretreatment of cells with 100 μg/mL groundnut extract prevented the depletion of glutathione by the radical initiator; however, treatment with 100 μg/mL of soybean isoflavone injured the cell membrane, indicating that glutathione might be released to the extracellular environment. These results suggest that the groundnut extract had isoflavone-like activity. Like soybean, groundnuts are a good source of isoflavones.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Apios Americana Medik; isoflavone; anti-oxidative stress; heme oxygenase-1 |
| Author Affiliation: | National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31 Midorigaoka, Ikeda, Osaka 563-8577, Japan, Tokyo Kasei-Gakuin University, Machida, Tokyo 194-0292, Japan, University of Occupational and Environmental Health, Japan |
| Subjects: | Postharvest Management Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Groundnut |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 Nov 2012 03:40 |
| Last Modified: | 20 Nov 2012 03:40 |
| Official URL: | http://dx.doi.org/10.1016/j.foodchem.2012.10.100 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8806 |
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