RFLP and RAPD mapping of the rice Gm2 gene that confers resistance to biotype 1 of gall midge (Orseolia oryzae).

Mohan, M. and Nair, S. and Bentur, J.S. and Rao, U.P. and Bennett, J. (1994) RFLP and RAPD mapping of the rice Gm2 gene that confers resistance to biotype 1 of gall midge (Orseolia oryzae). Theoretical and Applied Genetics, 87 ( 7 ). pp. 782-788.

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Abstract

Gm2 is a dominant gene conferring resistance to biotype 1 of gall midge, the major dipteran pest of rice. The gene was mapped by RFLP analysis of a set of 40 recombinant inbred lines derived from a cross between the rice resistant variety Phalguna and the susceptible landrace ARC6650. The gene is located on chromosome 4 at a position 1.3 cM from marker RG329 and 3.4 cM from RG476. Since the low (28%) polymorphism of this indica × indica cross hindered full coverage of the genome with RFLP markers, the mapping was checked by random amplified polymorphic DNA (RAPD)/bulked segregant analysis. Through the use of 160 RAPD primers, the number of polymorphic markers was increased from 43 to 231. Two RAPD primers amplified loci that co-segregated with resistance/susceptibility. RFLP mapping of these loci showed that they are located 0.7 cM and 2.0 cM from RG476, confirming the location of Gm2 in this region of chromosome 4. Use of these DNA markers will accelerate breeding for gall midge resistance by permitting selection of the Gm2 gene independently of the availability of the insect.

Item Type: Article
Additional Information: We thank Prof. K.K. Tewari, Dr. E.A. Siddiq and Dr. M. B. Kalode for their keen interest and support and the laboratory of S. D. Tanksley for probes. This research was supported in part by a grant from the Rockefeller Foundation to JB.
Uncontrolled Keywords: Oryza sativa, Orseolia oryzae, Gall midge Diptera, Bulked segregant analysis, Recombinant inbred lines, Insect resistance
Author Affiliation: International Centre for Genetic Engineering and Biotechnology, NII Campus, New Delhi.
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Arbind Seth
Date Deposited: 15 Nov 2012 04:07
Last Modified: 15 Nov 2012 04:07
Official URL: http://dx.doi.org/10.1007/BF00221129
URI: http://eprints.icrisat.ac.in/id/eprint/8767

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