Reddy, A.R. and Scheffler, B. and Madhuri, G. and Srivastava, M.N. and Kumar, A. and Sathyanarayanan, P.V. (1996) Chalcone synthase in rice (Oryza sativa L.): detection of the CHS protein in seedlings and molecular mapping of the chs locus. Plant Molecular Biology , 32 (4). pp. 735-743.
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Abstract
[Naringenin-]chalcone synthase (CHS) is a key enzyme that catalyses the first dedicated reaction of the flavonoid pathway in higher plants. The chs gene and its protein product in rice has been investigated. The presence of a chalcone synthase protein in rice seedlings and its developmental stage-specific expression was demonstrated by western analysis. The chalcone synthase of rice was immunologically similar to that of maize. A rice cDNA clone, Os-chs cDNA, encoding chalcone synthase, isolated from a leaf cDNA library of an indica rice variety Purpleputtu was mapped to the centromeric region of chromosome 11 of rice. It was mapped between RFLP markers RG2 and RG103. RG2 is the nearest RFLP marker located at a genetic distance of 3.3 cM. Some segments of chromosome 11 of rice including the chs locus are conserved on chromosome 4 of maize. The markers, including chs locus on chromosome 11 of rice are located, though not in the same order, on chromosome 4 of maize. Genetic analysis of purple pigmentation in two rice lines, Abhaya and Shyamala, used in the present mapping studies, indicated the involvement of three genes, one of which has been identified as a dominant inhibitor of leaf pigmentation. The Os-chs cDNA shows extensive sequence homology, both for DNA and protein (deduced), to that of maize, barley and also to different monocots and dicots.
| Item Type: | Article |
|---|---|
| Additional Information: | We thank Professor K. K. Tewari, Director, ICGEB, and Professor Udo Wienand, University of Hamburg, for their generous help and constant encouragement. Financial support from the Rockefeller Foundation to University of Hyderabad and International Centre for Genetic Engineering and Biotechnology (ICGEB) is gratefully acknowledged. Thanks are also due to the Directorate of Rice Research, Hyderabad for supply of plant materials. G.M. thanks the Department of Atomic Energy for providing a predoctoral research fellowship. We thank Dr Claude Fauquet, The Scripps Research Institute, California, for help with the phylogenetic analysis. |
| Uncontrolled Keywords: | Chalcone synthase cDNA (chs cDNA), RFLP, Anthocyanins, Flavonoids, Synteny |
| Author Affiliation: | International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi. |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 15 Nov 2012 04:53 |
| Last Modified: | 15 Nov 2012 04:53 |
| Official URL: | http://dx.doi.org/10.1007/BF00020214 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8765 |
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