Rajyashri, K.R. and Nair, S. and Ohmido, N. and Fukui, K. and Kurata, N. and Sasaki, T. (1998) Isolation and FISH mapping of yeast artificial chromosomes (YACs) encompassing an allele of the Gm2 gene for gall midge resistance in rice. Theoretical and Applied Genetics, 97 (4). 507-514.
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Abstract
Ten yeast artificial chromosomes (YACs) spanning the Gm2 locus were isolated by screening high-density filters containing a total of approximately 7000 YAC (representing six genome equivalents) clones derived from a japonica rice, cv. Nipponbare. The screening was done with five RFLP markers flanking a gall midge resistance gene, Gm2, which was previously mapped onto chromosome 4 of rice. This gene confers resistance to biotype 1 and 2 of gall midge (Orseolia oryzae), a major insect pest of rice in Southern and Southeast Asia. The RFLP markers RG214, RG329 and F8 hybridized with YAC Y2165. Two overlapping YAC clones (Y5212 and Y2165) were identified by Southern hybridization, with Gm2-flanking RFLP markers, and their inserts isolated. The purified YACs and RFLP markers flanking Gm2 were labelled and physically mapped by the fluorescence in situ hybridization (FISH) technique. All of them mapped to the long arm of chromosome 4 of the resistant variety of rice, Phalguna, confirming the previous RFLP mapping data.
| Item Type: | Article |
|---|---|
| Additional Information: | We thank Prof. K. K. Tewari, Director, ICGEB, for encouragement and support and Dr. K. Hasegawa, Managing Director, STAFF Institute, for facilities. We are thankful to Dr. J. Peacock, CSIRO, Australia for his suggestions and critical evaluation of the manuscript. This work was partially supported by funds from the Rockefeller Foundation, Ministry of Agriculture, Forestry and Fisheries (MAFF) of Japan and Japan Racing Association (JRA). |
| Uncontrolled Keywords: | Map-based cloning, Positional cloning, Fluorescence in situ hybridization, Disease resistance, Orseolia oryzae |
| Author Affiliation: | International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi. |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Arbind Seth |
| Date Deposited: | 15 Nov 2012 03:54 |
| Last Modified: | 15 Nov 2012 03:54 |
| Official URL: | http://dx.doi.org/10.1007/s001220050924 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8760 |
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