Chang, P.K. and Cary, J.W. and Bhatnagar, D. and et al, . (1993) Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis. Applied and Environmental Microbiology, 59 (10). pp. 3273-3279.
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Abstract
An Aspergillus parasiticus gene, apa-2, was identified as a regulatory gene associated with aflatoxin biosynthesis. Apa-2 gene cloning was based on the overproduction of pathway intermediates after transformation of fungal strs with cosmid DNA containing the aflatoxin biosynthetic genes nor-1 and ver-1. Transformation of an O-methylsterigmatocystin-accumulating str., A. parasiticus SRRC 2043, with a 5.5kb HindIII-XbaI DNA fragment containing apa-2 resulted in overproduction of all aflatoxin pathway intermediates analysed. Specific enzyme activities associated with the conversion of norsolorinic acid and sterigmatocystin were increased approx twofold. The apa-2 gene complemented an A. flavus afl-2 mutant str. for aflatoxin production, suggesting that apa-2 is functionally homologous to afl-2. Comparison of the A. parasiticus apa-2 gene DNA sequence with that of the A. flavus afl-2 gene indicated that they shared >85% DNA homology. Physical mapping of cosmid subclones placed apa-2 ~8kb from ver-1
| Item Type: | Article |
|---|---|
| Author Affiliation: | Southern Regional Research Center, ARS-USDA, 1100 Robert E. Lee Boulevard, New Orleans, LA 70124, USA |
| Subjects: | Soil Science and Microbiology > Microbiology Crop Improvement > Genetics/Genomics |
| Divisions: | Groundnut |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 12 Nov 2012 03:40 |
| Last Modified: | 12 Nov 2012 03:40 |
| Official URL: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC182448... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8738 |
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