Holzhauser, T. and Vieths, S. (1999) Indirect Competitive ELISA for Determination of Traces of Peanut (Arachis hypogaea L.) Protein in Complex Food Matrices. Journal of Agricultural and Food Chemistry, 47 (2). pp. 603-611.
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Abstract
An indirect competitive ELISA was developed allowing the detection of hidden peanut protein residues down to 2 ppm (micorgrams per gram) in various foods. The high-titer, peanut-specific polyclonal antiserum used recognized potentially allergenic proteins in both native and roasted peanuts. In the absence of a food matrix, extractable protein from roasted peanuts was detected at 104 ± 13%. From various food items, peanut protein at ≥13 ppm was recovered between 84 and 126%, and at 2 ppm of peanut protein recovery was 143 ± 6%. Intra- and interassay precision was <15%. In 5 of 17 commercial food products without declaration of peanut components, between 2 and 18 ppm of peanut protein was detected. This is the first assay based on commercially available reactants that allows the reliable determination of trace amounts of hidden peanut allergens in a variety of complex food matrices.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Peanut (Arachis hypogaea L.); hidden allergens; ELISA; immunoblotting |
| Author Affiliation: | Department of Allergology, Paul-Ehrlich-Institut, Paul-Ehrlich-Strasse 51-59, D-63225 Langen, Germany |
| Subjects: | Postharvest Management > Food Technology |
| Divisions: | Groundnut |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 06 Nov 2012 08:35 |
| Last Modified: | 06 Nov 2012 08:35 |
| Official URL: | http://dx.doi.org/10.1021/jf980775f |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8677 |
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