Expression of green fluorescent protein in Curvularia lunata causing maize leaf spot

Liu, T. and Liu, L. and Hou, J. and et al, . (2010) Expression of green fluorescent protein in Curvularia lunata causing maize leaf spot. Canadian Journal of Plant Pathology, 32 (2). pp. 225-228.

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Abstract

Curvularia lunata is a widespread fungal pathogen infecting maize leaves. In this study, we developed a genetic transformation system for C. lunata by using the plasmid (pCPXHY1Egfp) with both enhanced green fluorescent protein gene egfp and hygromycin resistance gene hph. GFP rendered hyphae of the transformants fluorescent in culture. Most transformants that showed constitutively expressing EGFP were comparable to the wild-type strain in morphological features. PCR and Southern blot analyses of the hph gene further confirmed that the plasmid was integrated into the genome of C. lunata. This is the first report that a ‘reporter’ gene, egfp, could be stably expressed in C. lunata. Using the transformants with EGFP marker will assist us to visualize the features of infection at different stages.

Item Type:	Article
Additional Information:	This work is supported by National Basic Research Program of China (973 Program, 2006 CB101901) and National Sci- ence & Technology Supporting Program of China (2006BAD08A06).
Uncontrolled Keywords:	Curvularia lunata, genetic transformation, green fluorescent protein
Author Affiliation:	School of Agriculture and Biology, Shanghai Jiaotong University, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, 800 Dongchuan Road, Shanghai, 200240, P.R. China, jiechen59@sjtu.edu.cn
Subjects:	Crop Improvement
Divisions:	General
Depositing User:	Mr Siva Shankar
Date Deposited:	02 Nov 2012 10:29
Last Modified:	02 Nov 2012 10:29
Official URL:	http://dx.doi.org/10.1080/07060661.2010.484213
URI:	http://eprints.icrisat.ac.in/id/eprint/8659

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