Kandasamy, S. and Loganathan, K. and Muthuraj, R.
(2009)
Understanding the molecular basis of plant growth promotional effect of Pseudomonas fluorescens on rice through protein profiling.
Proteome Science, 7.
8p.
Abstract
Plant Growth Promoting Rhizobacteria (PGPR), Pseudomonas fluorescens strain KH-1 was found to exhibit plant growth promotional activity in rice under both in-vitro and in-vivo conditions. But the mechanism underlying such promotional activity of P. fluorescens is not yet understood clearly. In this study, efforts were made to elucidate the molecular responses of rice plants to P. fluorescens treatment through protein profiling. Two-dimensional polyacrylamide gel electrophoresis strategy was adopted to identify the PGPR responsive proteins and the differentially expressed proteins were analyzed by mass spectrometry.
Results: Priming of P. fluorescens, 23 different proteins found to be differentially expressed in rice
leaf sheaths and MS analysis revealed the differential expression of some important proteins namely
putative p23 co-chaperone, Thioredoxin h- rice, Ribulose-bisphosphate carboxylase large chain
precursor, Nucleotide diPhosphate kinase, Proteosome sub unit protein and putative glutathione
S-transferase protein.
Conclusion: Functional analyses of the differential proteins were reported to be directly or
indirectly involved in growth promotion in plants. Thus, this study confirms the primary role of
PGPR strain KH-1 in rice plant growth promotion
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