Chen, H. and Wang, L. and Su, C.X. and et al, . (2008) Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Letters in Applied Microbiology, 47 (3). pp. 180-186.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png)
|
PDF
| Preview |
Abstract
Aims: Antibiotics from Bacillus subtilis JA show strong pathogen inhibition ability, which has potential market application; yet, the composition of these antibiotics has not been elucidated. The aim of this paper is to isolate and identify these antibiotics. Methods and Results: The antagonistic activity of JA was tested in vitro; it exhibited strong inhibition against some important phytopathogens and postharvest pathogens. Crude antibiotic production was extracted with methanol from the precipitate by adding 6 mol l−1 HCl to the bacillus-free culture broth. The crude extract was run on Diamonsil C18 column (5 μm, 250 × 4·6 mm) in HPLC system to separate the antibiotics. Major antibiotics were classified into three lipopeptide families according to electrospray ionization–mass spectrometry analysis. Subsequently, the classification of antibiotics was confirmed with typical collision-induced dissociation fragments. Conclusions: Three kinds of antibiotics were isolated from B. subtilis JA and were identified to the lipopeptide families, surfactin, iturin and fengycin. These compounds could function as biocontrol agents against a large spectrum of pathogens. Significance and Impact of the Study: This study provided a reliable and rapid method for isolation and structural characterization of lipopeptide antibiotics from B. subtilis
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Antibiotic; Bacillus subtilis; characterization; electrospray ionization mass spectrometry; lipopeptide |
| Author Affiliation: | Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui, China |
| Subjects: | Plant Protection Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Other Crops |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 29 Oct 2012 05:15 |
| Last Modified: | 29 Oct 2012 05:15 |
| Official URL: | http://dx.doi.org/10.1111/j.1472-765X.2008.02412.x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8556 |
Actions (login required)
 |
View Item |
