Geddes, C.C. and Mullinnix, M.T. and Nieves, I.U. and et al, . (2012) Seed Train Development for the Fermentation of Bagasse from Sweet Sorghum and Sugarcane using a Simplified Fermentation Process. Bioresource Technology. pp. 1-34.
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Abstract
A process was developed for seed culture expansion (3.6 million-fold) using 5% of the hemicellulose hydrolysate from dilute acid pretreatment as the sole organic nutrient and source of sugar. Hydrolysate used for seed growth was neutralized with ammonia and combined with 1.0 mM sodium metabisulfite immediately before inoculation. This seed protocol was tested with phosphoric acid pretreated sugarcane and sweet sorghum bagasse using a simplified process with co-fermentation of fiber, pentoses, and hexoses in a single vessel (SScF). A 6 h liquefaction (L) step improved mixing prior to inoculation. Fermentations (L+SScF process) were completed in 72 h with high yields (>80 gal/US ton). Ethanol titers for this L+SScF process ranged from 24 g/L to 32 g/L, and were limited by the bagasse concentration (10% dry matter).
| Item Type: | Article |
|---|---|
| Additional Information: | L.O. Ingram is a consultant for Myriant Corp. and a minor share holder. This research was supported by the Myriant Corp., the U.S. Department of Energy (DE-FG3608GO88142), and the U.S. Department of Agriculture (2011-10006-30358). |
| Uncontrolled Keywords: | Biomass; Lignocellulose; SScF; L+SScF; Ethanol; Xylose |
| Author Affiliation: | University of Florida, Box 110700, Gainesville, C.E. Geddes, Novozymes, Box 576, 77 Perry’s Chapel Church Road,Franklinton, NC 27525 |
| Subjects: | Crop Improvement Plant Physiology and Biochemistry > Plant Physiology |
| Divisions: | Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 Oct 2012 05:08 |
| Last Modified: | 20 Oct 2012 05:08 |
| Official URL: | http://dx.doi.org/10.1016/j.biortech.2012.09.121 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8470 |
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