Conjugation of Antibodies to Alkaline Phosphatase

Wisdom, G.B. (2005) Conjugation of Antibodies to Alkaline Phosphatase. In: Immunochemical Protocols. Methods in Molecular Biology (295). Humana Press, USA, pp. 123-126. ISBN 978-1-59259-873-1

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Abstract

Alkaline phosphatase is coupled to immunoglobin G antibody in a one-step procedure using the homobifunctional reagent glutaraldehyde, which reacts with amino groups in the two proteins. The procedure is simple to perform and requires minimal equipment.

Item Type:	Book Section
Uncontrolled Keywords:	Antibody, alkaline phosphatase, conjugation, labeling, glutaraldehyde
Author Affiliation:	School of Biology and Biochemistry, The Queens University, Belfast, Northern Ireland, UK
Subjects:	Crop Improvement
Divisions:	General
Depositing User:	Mr Siva Shankar
Date Deposited:	19 Oct 2012 05:28
Last Modified:	19 Oct 2012 05:28
Official URL:	http://link.springer.com/protocol/10.1385%2F1-5925...
URI:	http://eprints.icrisat.ac.in/id/eprint/8466

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