Calarco, J.P. and Borges, F. and Donoghue, M.T.A. and et al, . (2012) Reprogramming of DNA Methylation in Pollen Guides Epigenetic Inheritance via Small RNA. Cell, 151. pp. 194-205.
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Abstract
Epigenetic inheritance is more widespread in plants than in mammals, in part because mammals erase epigenetic information by germline reprogramming. We sequenced the methylome of three haploid cell types from developing pollen: the sperm cell, the vegetative cell, and their precursor, the postmeiotic microspore, and found that unlike in mammals the plant germline retains CG and CHG DNA methylation. However, CHH methylation is lost from retrotransposons in microspores and sperm cells and restored by de novo DNA methyltransferase guided by 24 nt small interfering RNA, both in the vegetative nucleus and in the embryo after fertilization. In the vegetative nucleus, CG methylation is lost from targets of DEMETER (DME), REPRESSOR OF SILENCING 1 (ROS1), and their homologs, which include imprinted loci and recurrent epialleles that accumulate corresponding small RNA and are premethylated in sperm. Thus genome reprogramming in pollen contributes to epigenetic inheritance, transposon silencing, and imprinting, guided by small RNA.
| Item Type: | Article |
|---|---|
| Additional Information: | This work was supported by NIH grant R01 GM067014 to R.A.M., grants PTDC/AGR-GPL/103778/2008 and PTDC/ BIA-BCM/103787/2008 from Fundac¸ a˜ o para a Cieˆ ncia e a Tecnologia (FCT) to J.D.B. and FCT grant PTDC/BIA-BCM/108044/2008 to J.A.F. F. Berger and P.E.J. were supported by Temasek Lifescience Laboratory. J.P.C. was supported by a graduate student fellowship from NSERC and by a grant from the Fred C. Gloeckner Foundation, F. Borges was supported by a FCT PhD fellowship SFRH/BD/48761/2008, and F.V.E. was funded by a Herbert Hoover postdoctoral fellowship from the Belgian American Educational Foundation. |
| Uncontrolled Keywords: | DNA, RNA |
| Author Affiliation: | Howard Hughes Medical Institute-Gordon and Betty Moore Foundation, Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA |
| Subjects: | Crop Improvement > Genetics/Genomics |
| Divisions: | General |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 16 Oct 2012 03:13 |
| Last Modified: | 16 Oct 2012 03:13 |
| Official URL: | http://dx.doi.org/10.1016/j.cell.2012.09.001 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8449 |
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