Exploration and characterization of agriculturally and industrially important haloalkaliphilic bacteria from environmental samples of hypersaline Sambhar lake, India

Sahay, H. and Mahfooz, S. and Singh, A.K. and et al, . (2012) Exploration and characterization of agriculturally and industrially important haloalkaliphilic bacteria from environmental samples of hypersaline Sambhar lake, India. World Journal of Microbiology and Biocechonology, 28 (1). pp. 3207-3217.

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Abstract

Screening of bacteria from Sambhar lake, an extreme hypersaline environment of India, led to the isolation of 93 haloalkaliphilic bacteria growing optimally in media with 2-25 % salt and 6-12 pH. Based on 16S rRNA gene sequences, 93 isolates were further categorized into 32 groups, with each group representing a different taxa belonging to 3 phyla (Firmicutes, Proteobacteria and Actinobacteria). Majority of the isolates (53.12 %) showed similarity with phylum Firmicutes which was followed by Proteobacteria (40.63 %) and Actinobacteria (6.25 %). The isolates belonging to 32 representative groups were further evaluated for the production of extracellular enzymes viz. amylase, cellulase, protease and xylanase, plant growth promoting attributes and BIOLOG™ substrate usage. Among all the isolates, xylanase producing isolates were in maximum (68 %) as compared to protease (56 %), cellulase (40 %), and amylase (37 %) producing strains. Similarly, among plant growth promoting activities, ammonia producing isolates were highest (56 %) when compared to those producing ACC deaminase (53 %), IAA (50 %), hydrogen cyanide (28 %), siderophore (21 %) and solubilizing P (34 %). Isolates showing enzymatic and PGP activities could be further utilized for promoting plant growth in saline affected area

Item Type: Article
Author Affiliation: National Bureau of Agriculturally Important Microorganisms, Kusmaur, Mau, 275101, Uttar Pradesh, India
Subjects: Soil Science and Microbiology > Microbiology
Divisions: General
Depositing User: Ms K Syamalamba
Date Deposited: 15 Oct 2012 03:39
Last Modified: 15 Oct 2012 03:39
Official URL: http://www.ncbi.nlm.nih.gov/pubmed/22828794
URI: http://eprints.icrisat.ac.in/id/eprint/8444

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