Advances in targeted genome editing

Perez-Pinera, P. and Ousterout, D.G. and Gersbach, C.A. (2012) Advances in targeted genome editing. Current Opinion in Chemical Biology, 16. pp. 268-277.

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Abstract

New technologies have recently emerged that enable targeted editing of genomes in diverse systems. This includes precise manipulation of gene sequences in their natural chromosomal context and addition of transgenes to specific genomic loci. This progress has been facilitated by advances in engineering targeted nucleases with programmable, site-specific DNAbinding domains, including zinc finger proteins and transcription activator-like effectors (TALEs). Recent improvements have enhanced nuclease performance, accelerated nuclease assembly, and lowered the cost of genome editing. These advances are driving new approaches to many areas of biotechnology, including biopharmaceutical production, agriculture, creation of transgenic organisms and cell lines, and studies of genome structure, regulation, and function. Genome editing is also being investigated in preclinical and clinical gene therapies for many diseases.

Item Type: Article
Additional Information: This work was supported by The Hartwell Foundation, a Basil O’Connor Starter Scholar Award from the March of Dimes, an NSF Faculty Early Career Development (CAREER) Award (1151035) and an NIH Director’s New Innovator Award (1DP2OD008586).
Uncontrolled Keywords: Genome Editing. DNA
Author Affiliation: Department of Biomedical Engineering, Duke University, Durham, NC 27708-0281, USA
Subjects: Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 12 Oct 2012 06:15
Last Modified: 12 Oct 2012 06:15
Official URL: http://dx.doi.org/10.1016/j.cbpa.2012.06.007
URI: http://eprints.icrisat.ac.in/id/eprint/8410

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