Perl, S. (1978) Phosphoenol-pyruvate-carboxylase activity in cotton and Sorghum seeds and its relation to seedling development. Planta, 139 (3). pp. 239-243.
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Abstract
Cotton and sorghum seeds incorporated CO2 through a PEP-carboxylating enzyme. Enzyme activity was opt. at pH 8.2 and was unaffected by ATP, GDP or acetyl CoA. Partially purified cotton enzyme was stimulated by inorganic phosphate with an apparent Km of 0.3 mM. Enzymes from both spp. were inhibited by pyrophosphate, malate and aspartate but not by succinate. Kinetic studies for sorghum and cotton seed enzymes showed apparent Km values for carbonate of 5 mM and 1.2 mM and for PEP of 36 mu M and 5 mM, resp. The Vmax values were 90 and 3.3 nmol/min mg protein, resp. A 2-fold increase in enzyme activity from cotton seeds occurred after 2 h under laboratory germination conditions after which the activity dropped sharply to 33% of the original activity after 5 h of imbibition. No such change was observed in sorghum seed enzyme. PEP-carboxylase activity and seed vigour was correlated in both spp
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Gossypium; PEP-carhoxylase; Seed vigor; Sorghum |
| Author Affiliation: | Division of Seed Research, Volcani Center, P.O.B.6, Bet Dagan, Israel 50200 |
| Subjects: | Plant Production Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 10 Oct 2012 03:53 |
| Last Modified: | 10 Oct 2012 03:53 |
| Official URL: | http://dx.doi.org/10.1007/BF00388635 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8346 |
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