Kannangara, T and Durley, R.C. and Simpson, G.M. (1978) High performance liquid chromatographic analysis of cytokinins in sorghum bicolor leaves. Physiologia Plantarum, 44 (3). pp. 295-299.
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Abstract
High performance liquid chromatography with octadecylsilica (Bondapak C18/Poracil B) column packing was used to purify and separate cytokinins in Sorghum leaf extracts. The column size was 56 × 0.21 cm i.d. By gradient elution, using acidified water with increasing amounts of methanol, the major peaks of cytokinin activity, as determined by the callus tissue bioassay. were effectively separated from large amounts of extraneous impurities. These cytokinins were further separated on a microoctadecylsilica column (μBondapak C18, 30 × 0.4 cm i.d.) with a gradient of acidified water-acetonitrile. Zeatin and zeatin riboside gave distinct ultra violet absorption peaks which could be used for quantitative estimation. Biological activity corresponded to the elution of these peaks. These two cytokinins are the major cytokinins in Sorghum leaves
| Item Type: | Article |
|---|---|
| Author Affiliation: | Crop Science Department, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N 0W0 |
| Subjects: | Plant Physiology and Biochemistry > Plant Physiology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 08 Oct 2012 09:21 |
| Last Modified: | 10 Oct 2012 10:39 |
| Official URL: | http://dx.doi.org/10.1111/j.1399-3054.1978.tb08635... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8323 |
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