Stability of nitrate reductase and source of its reductant in Sorghum seedlings

Kadam, S.S. and Johari, R.P. and Ramarao, C.S and Srinivasan, C.S. (1980) Stability of nitrate reductase and source of its reductant in Sorghum seedlings. Phytochemistry, 19 (10). pp. 2095-2097.

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Abstract

Pre-incubation of nitrate from sorghum seedlings with NADH increased enzyme activity by 25%. Ferricyanide had no effect. NADH protected the enzyme from inactivation during storage. Malonate inhibited in vivo nitrate reduction in sorghum leaves by 95%. The inhibitory effect of malonate was reversed by fumarate. NaF in the presence of phosphate also inhibited in vivo nitrate reduction by 60%. It was suggested that NADH generated via the citric acid cycle is utilized for nitrate reduction in sorghum seedlings

Item Type: Article
Author Affiliation: Division of Biochemistry, Indian Agricultural Research Institute, New Delhi 110 012, India
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Plant Physiology and Biochemistry > Biochemistry
Divisions: Sorghum
Soyabean
Depositing User: Ms K Syamalamba
Date Deposited: 08 Oct 2012 08:04
Last Modified: 08 Oct 2012 08:04
Official URL: http://dx.doi.org/10.1016/S0031-9422(00)82200-1
URI: http://eprints.icrisat.ac.in/id/eprint/8320

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