Analysis of abscisins and 3-indolylacetic acid in leaves of Sorghum bicolor by high performance liquid chromatography

Durley, R.C. and Kannangara, T and Simpson, G.M. (1978) Analysis of abscisins and 3-indolylacetic acid in leaves of Sorghum bicolor by high performance liquid chromatography. Canadian Journal of Botany, 56 (2). pp. 157-161.

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Abstract

ABA, phaseic acid, dihydrophaseic acid and IAA contents in leaf extracts of sorghum were purified in 37- to 75- mu m diam. octadecylsilica columns and analysed with microoctadecylsilica columns, both used in the reverse phase mode. The efficiency and resolution of the microcolumn combined with high sensitivity of the UV absorption microcell detector could accurately determine as little as 2 ng of ABA and 6 ng of IAA. The method was considered to be suitable for routine analysis of growth regulator contents in moderately large numbers of plant extracts

Item Type: Article
Author Affiliation: Department of Crop Science, Saskatchewan University, Saskatoon, Saskatchewan, Canada S7N 0W0
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 08 Oct 2012 03:40
Last Modified: 08 Oct 2012 03:40
Official URL: http://dx.doi.org/10.1139/b78-019
URI: http://eprints.icrisat.ac.in/id/eprint/8305

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