Dunstan, D.I. and Short, K.C. and Thomas, E. (1978) The anatomy of secondary morphogenesis in cultured scutellum tissues of Sorghum bicolor. Protoplasma, 92 (2-3). pp. 251-260.
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Abstract
Cells of the scutellum of immature embryos of sorghum plated onto an agar medium containing 2,4-D gave rise to shoots and embryo-like structures and to some callus. Some of the embryo-like structures later developed into typical sorghum embryos complete with scutellum, coleoptile and coleorhiza. The results of anatomical studies of the development of these secondary growth forms by light and scanning electron microscopy suggest that shoots and embryo-like structures can arise directly from cells of the primary scutellum without an intervening callus phase. In some cases it appears that the scutellum of the secondary embryos arises by folding of the scutellum of the sexual embryo and does not arise de novo. In other cases the structures arise from single cells. No evidence was found to indicate that organized structures arose from proliferating callus cells. The unorganized callus which arises initially is not capable of growth through continuous subculture; it produces a purple-black pigment and rapidly becomes necrotic. The significance of these observations is discussed in relation to present views on morphogenesis in cereal cell cultures and their implications for in vitro cell genetics
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biology, Division of Plant Sciences, North East London Polytechnic, London E15 4LZ, UK |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Ms K Syamalamba |
| Date Deposited: | 05 Oct 2012 10:49 |
| Last Modified: | 05 Oct 2012 10:49 |
| Official URL: | http://dx.doi.org/10.1007/BF01276697 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/8290 |
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